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penicillin streptomycin p s  (ATCC)


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    ATCC penicillin streptomycin p s
    Penicillin Streptomycin P S, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+skeletal+muscle+growth+kit/Primary+Skeletal+Muscle+Growth+Kit/pmc13091133-72-33-30
    Average 94 stars, based on 29 article reviews
    penicillin streptomycin p s - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells
    Article Snippet: .. HSkMCs (ATCC, Manassas, VA, USA) were cultured according to the manufacturer's recommendations in ATCC Mesenchymal Stem Cell Basal Medium (PCS-500-030, ATCC) supplemented with the Primary Skeletal Muscle Growth Kit (PCS-950-040, ATCC) and 1% penicillin–streptomycin (P/S), under standard culture conditions (37 °C, 5% CO 2 ). .. In parallel, hASCs (Lonza, Basel, Switzerland) were cultured in DMEM/F-12 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal calf serum (FCS) and 1% P/S under the same conditions.

    Article Title: microRNA-22 Inhibition Stimulates Mitochondrial Homeostasis and Intracellular Degradation Pathways to Prevent Muscle Wasting
    Article Snippet: .. HSkMC was cultured in a Primary Skeletal Muscle Growth Kit (ATCC, Cat. No. PCS-950-040, Manassas, VA, USA). .. Cells were allowed to differentiate on fibronectin-coated plates in Skeletal Muscle Differentiation Medium (ATCC, Cat. No. PCS-950-050) for 5 days.

    Article Title: Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells
    Article Snippet: .. HSkMCs (ATCC, Manassas, VA, USA) were cultured according to the manufacturer's recommendations in ATCC Mesenchymal Stem Cell Basal Medium (PCS-500-030, ATCC) supplemented with the Primary Skeletal Muscle Growth Kit (PCS-950-040, ATCC) and 1% penicillin–streptomycin (P/S), under standard culture conditions (37 ◦C, 5% CO2). .. In parallel, hASCs (Lonza, Basel, Switzerland) were cultured in DMEM/F-12 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal calf serum (FCS) and 1% P/S under the same conditions.

    Modification:

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: HepG2 cells (ATCC Cat# HB-8065, RRID:CVCL_0027, https://scicrunch.org/resolver/RRID:CVCL_0027 ) Human primary skeletal muscle cells (HSkMCs; ATCC Cat# PCS-950–010) IMR-90 cells (ATCC Cat# CCL-186, RRID:CVCL_0347, https://scicrunch.org/resolver/RRID:CVCL_0347 ) CAUTION: The cell lines used should be regularly checked to ensure they are authentic and are not infected with mycoplasma. .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup . ..

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) .. Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup .

    Sterility:

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: HepG2 cells (ATCC Cat# HB-8065, RRID:CVCL_0027, https://scicrunch.org/resolver/RRID:CVCL_0027 ) Human primary skeletal muscle cells (HSkMCs; ATCC Cat# PCS-950–010) IMR-90 cells (ATCC Cat# CCL-186, RRID:CVCL_0347, https://scicrunch.org/resolver/RRID:CVCL_0347 ) CAUTION: The cell lines used should be regularly checked to ensure they are authentic and are not infected with mycoplasma. .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup . ..

    Transferring:

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: HepG2 cells (ATCC Cat# HB-8065, RRID:CVCL_0027, https://scicrunch.org/resolver/RRID:CVCL_0027 ) Human primary skeletal muscle cells (HSkMCs; ATCC Cat# PCS-950–010) IMR-90 cells (ATCC Cat# CCL-186, RRID:CVCL_0347, https://scicrunch.org/resolver/RRID:CVCL_0347 ) CAUTION: The cell lines used should be regularly checked to ensure they are authentic and are not infected with mycoplasma. .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup . ..

    Microscopy:

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: HepG2 cells (ATCC Cat# HB-8065, RRID:CVCL_0027, https://scicrunch.org/resolver/RRID:CVCL_0027 ) Human primary skeletal muscle cells (HSkMCs; ATCC Cat# PCS-950–010) IMR-90 cells (ATCC Cat# CCL-186, RRID:CVCL_0347, https://scicrunch.org/resolver/RRID:CVCL_0347 ) CAUTION: The cell lines used should be regularly checked to ensure they are authentic and are not infected with mycoplasma. .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup . ..

    Fluorescence:

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: HepG2 cells (ATCC Cat# HB-8065, RRID:CVCL_0027, https://scicrunch.org/resolver/RRID:CVCL_0027 ) Human primary skeletal muscle cells (HSkMCs; ATCC Cat# PCS-950–010) IMR-90 cells (ATCC Cat# CCL-186, RRID:CVCL_0347, https://scicrunch.org/resolver/RRID:CVCL_0347 ) CAUTION: The cell lines used should be regularly checked to ensure they are authentic and are not infected with mycoplasma. .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup . ..

    Software:

    Article Title: An explainable deep learning platform for molecular discovery
    Article Snippet: HepG2 cells (ATCC Cat# HB-8065, RRID:CVCL_0027, https://scicrunch.org/resolver/RRID:CVCL_0027 ) Human primary skeletal muscle cells (HSkMCs; ATCC Cat# PCS-950–010) IMR-90 cells (ATCC Cat# CCL-186, RRID:CVCL_0347, https://scicrunch.org/resolver/RRID:CVCL_0347 ) CAUTION: The cell lines used should be regularly checked to ensure they are authentic and are not infected with mycoplasma. .. Liquid LB medium (Becton Dickinson 244620) LB media containing 1.5% (wt/vol) Difco agar (Becton Dickinson 244520) Dulbecco’s Modified Eagle Medium (DMEM; Corning 10–013-CV) Fetal bovine serum (FBS; ThermoFisher 16140071) Penicillin-streptomycin (PS; ThermoFisher 15070063) Mesenchymal stem cell basal medium for adipose, umbilical and bone marrow-derived MSCs (ATCC PCS-500–030) Primary skeletal muscle growth kit (ATCC PCS-950–040) Eagle’s Minimum Essential Medium (EMEM; ATCC 30–2003) Trypsin-EDTA, 0.05% (wt/vol) (Corning) Resazurin (MilliporeSigma R7017) Pharmakon Collection (MicroSource Discovery Systems) Natural Products Collection (MicroSource Discovery Systems) Optional: dimethyl sulfoxide (DMSO; MilliporeSigma D5879) Sterile pipette tips (Rainin) Optional: liquid-handling robot (e.g., Agilent Bravo or similar) Sterile microbiological inoculation loops (Beckton Dickinson) Sterile 14 mL culture tubes (Falcon) Sterile 15 mL centrifuge tubes (Falcon) Sterile untreated clear flat-bottom 96-well plates (Corning) Sterile tissue-treated black flat-bottom 96-well plates (Corning) Sterile untreated petri dishes, 100 mm × 15 mm (Falcon) Sterile tissue culture flasks, T-175 (Falcon) Sterile reagent reservoirs (Corning) Sterile filter, 0.45 μm (Corning) Microbiological humidity-controlled incubator set to 37°C and 0% CO 2 (vol/vol), with shaking option (ThermoFisher) Tissue culture humidity-controlled incubator set to 37°C and 5% CO 2 (vol/vol) (ThermoFisher) Inverted tissue culture microscope with a low-magnification (e.g., 4×) objective (Leica) Plate reader capable of reading absorbance and fluorescence (e.g., SpectraMax M3 plate reader or similar) Centrifuge with rotors for Falcon tubes and plates Optional: Liquid dispenser with sterile cassette (e.g., Agilent BioTeK MultiFlo dispenser or similar) Internet connection is required to download the software in Equipment setup . ..



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    Image Search Results


    Effect of rADAMTS-1 on MuSCs following BaCl 2 -induced TA muscle injury. Groups included NC, BaCl 2 , and two treatment groups receiving rADAMTS-1 (L) and (H). Daily intraperitoneal injections of rADAMTS-1 were initiated immediately after injury and continued until the designated time point for analysis. (A) Flow cytometry analysis of MuSCs isolated from injured TA muscles, defined by surface marker expression (CD45 − /CD31 − /Sca1 − /VCAM1 + ). (B) Representative gating strategy for MuSCs across experimental groups at different time points. (C) Relative number of MuSCs normalized to the NC group over time. Statistical significance was determined using one-way analysis of variance, followed by Tukey's post hoc test: * P<0.05, ** P<0.01 and *** P<0.001 vs. NC; ## P<0.01 and ### P<0.001 vs. BaCl 2 group. (D) Representative immunofluorescence images of Pax7 + cells in injured TA muscle at different time points (scale bar, 25 μ m). (E) Quantification of Pax7 + cell percentages over time. Statistical significance was determined using one-way analysis of variance with Tukey's post hoc test: * P<0.05 and *** P<0.001 vs. NC; ### P<0.001 vs. BaCl 2 ; ††† P<0.001 vs. rADAMTS-1 (L). ADAMTS-1, a disintegrin and metalloproteinase with thrombospondin motifs 1; rADAMTS-1, recombinant ADAMTS-1; MuSCs, skeletal muscle satellite cells; BaCl 2, barium chloride; TA, tibialis anterior; NC, non-injured control; rADAMTS-1 (L), rADAMTS-1 at 5 mg/kg; rADAMTS-1 (H), rADAMTS-1 at 10 mg/kg; CD, cluster of differentiation; Sca1, stem cell antigen 1; VCAM1, vascular cell adhesion molecule; Pax7, paired box protein.

    Journal: International Journal of Molecular Medicine

    Article Title: Recombinant ADAMTS-1 promotes muscle regeneration accompanied by downregulation of Notch signaling

    doi: 10.3892/ijmm.2025.5718

    Figure Lengend Snippet: Effect of rADAMTS-1 on MuSCs following BaCl 2 -induced TA muscle injury. Groups included NC, BaCl 2 , and two treatment groups receiving rADAMTS-1 (L) and (H). Daily intraperitoneal injections of rADAMTS-1 were initiated immediately after injury and continued until the designated time point for analysis. (A) Flow cytometry analysis of MuSCs isolated from injured TA muscles, defined by surface marker expression (CD45 − /CD31 − /Sca1 − /VCAM1 + ). (B) Representative gating strategy for MuSCs across experimental groups at different time points. (C) Relative number of MuSCs normalized to the NC group over time. Statistical significance was determined using one-way analysis of variance, followed by Tukey's post hoc test: * P<0.05, ** P<0.01 and *** P<0.001 vs. NC; ## P<0.01 and ### P<0.001 vs. BaCl 2 group. (D) Representative immunofluorescence images of Pax7 + cells in injured TA muscle at different time points (scale bar, 25 μ m). (E) Quantification of Pax7 + cell percentages over time. Statistical significance was determined using one-way analysis of variance with Tukey's post hoc test: * P<0.05 and *** P<0.001 vs. NC; ### P<0.001 vs. BaCl 2 ; ††† P<0.001 vs. rADAMTS-1 (L). ADAMTS-1, a disintegrin and metalloproteinase with thrombospondin motifs 1; rADAMTS-1, recombinant ADAMTS-1; MuSCs, skeletal muscle satellite cells; BaCl 2, barium chloride; TA, tibialis anterior; NC, non-injured control; rADAMTS-1 (L), rADAMTS-1 at 5 mg/kg; rADAMTS-1 (H), rADAMTS-1 at 10 mg/kg; CD, cluster of differentiation; Sca1, stem cell antigen 1; VCAM1, vascular cell adhesion molecule; Pax7, paired box protein.

    Article Snippet: Primary skeletal muscle cells (cat. no. PCS-950-010; ATCC) were cultured in a complete expansion medium (cat. no. PCS-500-030; ATCC) using a primary skeletal muscle cell growth kit (cat. no. PCS-950-040; ATCC).

    Techniques: Flow Cytometry, Isolation, Muscles, Marker, Expressing, Immunofluorescence, Recombinant, Control